bglA [2019-08-13 10:00:36]

6-phospho-beta-glucosidase

locus: BSU_40110

pI: 4.65

mw: 54.67 kDa

protein length: 479 aa

gene length: 1440 bp

function: beta-glucoside utilization

product: 6-phospho-beta-glucosidase)

essential: no

ec: 3.2.1.86

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.18|Utilization of beta-glucosides]

Gene

Coordinates: 4,121,166 4,122,605

The protein

Catalyzed reaction/ biological activity

• 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

Protein family

• [SW|glycosyl hydrolase 1 family] (according to UniProt)

Structure

• [PDB|2XHY] (from E. coli, 66% identity) [pubmed|22393408]

Expression and Regulation

Operons

• genes: [gene|FF3C9A092A3CAE0EB808F9C615BF5FC433C17E81|yyzE]-[gene|6A06E0E3017DB6E75D5C1114D954D51CED546051|bglA]
  description: [pubmed|22383849]

Biological materials

Mutant

• BKE40110 ([gene|6A06E0E3017DB6E75D5C1114D954D51CED546051|bglA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCATGTTTAATT, downstream forward: _UP4_TAAGAAGCGCTCGAATCGAG
• BKK40110 ([gene|6A06E0E3017DB6E75D5C1114D954D51CED546051|bglA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCCCATGTTTAATT, downstream forward: _UP4_TAAGAAGCGCTCGAATCGAG

References

• 14652714,15139916,10627040,8125345